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Drosophila Gene in Drosophila Melanogaster Presentation

by | Sep 4, 2021 | Science

Description


Powerpoint

Presentation Guide

Slide 1- Title page

Slide 2- Chromosome your gene is located on and how many splice variants is has.

Slide 3- Tops results of your blast search results and PFAM results

Slide 4- Function of your gene according to your research

Slide 5- The Crispr site you decided on

Slide 6- Describe the different amplicon you designed

Slide 7- Describe the primer pair you picked to screen for modifications

Slide 8 – Describe interpretation of your fragment analysis

Slide 9- Describe if you got a stop codon or not

Slide 10- Protein models mutant vs. wild-type

Slide 11- Fluorescence and Attp were also generated for marking and modifying gene

Slide 12 – Experimental Design to test your hypothesis

Slide 13- Data and Interpretation

Gene editing of Orco homolog in Aedes aegypti
Gene was located on chromosome 3 and contained only 1 splice variant
“AAEL005776” is a homolog of Orco and is has 7-TM domains
7-transmembrane Odorant receptor
Aedes Albopictus
Drosophila melanogaster
Blastp
Odorant receptor coreceptor
Query Cover :100%
Per Ident: 98.12%
Tblastn
Gene:Orco
Query Cover: 99
Per Ident: 72.52
Orco homolog function in D. melanogaster
Orco is a universal and integral part of the functional odorant receptor,
involved in the dendritic localization of other olfactory receptors. ]
Created a target on Exon 2
Generated Primers to amplify region
F1-R1:373
F1-R2:348
F2-R1:343
F2-R2:317
Selected Primers to amplify region
373bp WT
300
Flourecence
Fluorescence
Fluorescence
Fluorescence
Flourecence
Flourecence
Fragment analysis of 10bp insertion
310
320
330
340
350
360
370
380
Molecular weight (bp)
Heterozygote mutant
390
400
300
310
320
330
340
350
360
370
380
390
Molecular weight (bp)
Homozygote wild-type
400
300
310
320
330
340
350
360
370
380
390
Molecular weight (bp)
Homozygote mutant
400
Insertions results in premature stop codon
Exon 2 open reading frame
# 5’3′ Frame 1
MNVQPTKYHGLVLDLMPNIRLMQGFGHFLFRYVNGPVLIRKLYSWWNLIMILLQYFAIMGNLVMN
TGDVNELTANTITTLFFTHSVTKFIYVAVNSEHFYRTLGIWNQPNSHSLFAESDARYHSIALAKMRKLL
VMVMVTTVLSVV
Exon 2 closed reading frame after insertion
MNVQPTKYHGLVLDLMPNIRLMQGFGHFLFRYVNGPVLIRKLYSIINMVEPDNDPAAVFCHHGQPGDEYRGRQANGKHHNDAVFHPFRDQVHLRRRQLGTFLPHAGHLESTEQSFTFCRIGCSVPFDCVG-DAKTAGHGDGDYSAIRR
Protein model indicate potential truncated protein
Wt Orco protein 3D model
Possible truncated Orco protein 3D model
Designed Fluorescence marker construct and attp insertion for HDR orco mutants
Homologous L
3xP3
dsRed
SV40
Homologous R
Experimental Design
Two-port olfactometer assays. The olfactometer, consisted of a large plexiglass box (50 x 50 x 80 cm) connected to two
smaller cylindrical traps at one end (18 cm long, 9 cm inner diameter), which were in turn connected to two stimulus ports.
The opposite end of the main compartment was covered with a screen and a spongy mesh filter, and then connected to a
box fan. When on, the box fan pulled air through the stimulus ports where it mixed with the stimuli, then through the traps
and into the main compartment.
Ten min prior to the start of the assay, mosquitoes were released into the main compartment to acclimate. At the end of the
acclimation period, the fan was turned on and a sliding door between the trapping chambers and main compartment was
opened to allow air to flow through the apparatus. The number of mosquitoes in each trap was counted after 8 min.
Orco is involved in honey detection
10
Text map of attp insert
Final choice for uorescent insert
Snapshot of 3 pairs of restriction enzymes
Snapshot of completed activity on slide 30
Snapshot of completed activity on slide 31
Graphic map
Text Map
Primer pair length wild- type and mutant and Screenshot GEL
Screenshot of +/+, mu/+, mu/mu fragment analysis
1. Poster of chromosome
2. Poster of gene structure
3
Coding sequence le
>AAEL005039:AAEL005039-RB cds:protein_coding
ATGCACACATCTACCAGAGGATGGATGGTCAGGTTTTTCCTGATAAGCAGTTTCTTGTCA
ACGCTGGTTGAATCCAAACCAGCGACTAATCGTTCCAATTTTGACGACGATAATGACCTC
GCCGATTTCGGGCACCTTTTGATCGATCTGGTTGCCAAGATGAAGGCTGGCCATTGCTAT
GCCTTCATCACGGATAATATCTACCAGGTTGTGTTAACGGGTCGTCTCTTCCGCGAGATT
GAAACCTTTCCACGTTACGTAGTTCAAATTCCGGACTATGAAGACACGCTAGATCCCGGA
ACACGTGTCCGGTGCATGCTCGAGGAAGTTCGGAAAATCGGTTGCGGGGCCTATGTTATA
TTGCTGACCAATGGGATCCAGATGGAGCGGTTGTTACGCTTTGGGGATAGGACAAGGATA
ATCAATACTCGCGCAAAGTTCATCATTCTCCACGACTATCGCCTGTTCGTGCCTGACCTG
CATTTTCTGTGGAAACGGATAGTAAATGTTGTTTTCGTCAGACAGCTGGTGATTGACAAA
GCCAAGAAAAGGCATGAAAGCCACCACTACGAACTAAGCACCGTTCCGTTTCCGTTCCCC
TTGAGAGGAGTGTTTATTTCGCGAAGGCTCGATTTTTGGTTCAACGGAAAATTTCGAAAA
GGCCGAAAACTGTTCTCGGATAAGACCACTAACTTGGACGGACAATCGATGAAGGTCGTA
GTGCTGGAGCATACACCGGCGATATTCAGAACCACCCACAATGAGACGGACGAGCATTTG
AAGTACTACGGGTTGGAAGTGGAGCTGCTCAAAGCGATTTCCGAAGCAATGAAATTTGAA
ATGGATTTCTACGAAAGCGATGATGCAGCCGTTGCCATGTGGGGTACCGTAACAGACGGC
GAAAATGCTACAGGATTACTCGGAGAAATGAATGAAGGCCACGCTGACTTTGCGTTGGCT
GATCTCCACCACACGCAATACCACTTGGAAATCATGGACCTGAGCATCCCGTACAACACC
GAGTGTCTGACGTTCCTGACTCCGGAAGCGCTGACCGACAATTCTTGGACGACGCTAATA
CTGCCTTTCACCGGAGGAATGTGGGCTGGCGTCCTGGCATCGCTGTTTTCGATCGGAACC
GTATTCTATGCCCTGTCGAGGCTTCTCATGTTCGTACGCCACGAACAATCCTATCGCAGC
GACTTGGATATGATTGCAAAAAGAAGTAGAAAACGCAATCGGAAAGTTCATTTCAAGATA
GTTTGTGGTATGGTGAATAGCTATAAGAGAGCACTGGGTAACCGGAAATGGTTGAAGTTC
AAGAAGAAATCGACGGATACCTCAAATATGAACAAACTGAAAATGCTCAAAATGATTCCA
TTCAAAAGACAAGCTCTTCCTTGGAAAGATCCCCTGCCTCCCCGGGACATATTTGATACT
TTCTCTGGATGTATCATCTATACGTATAGTATGCTTTTGCTAGTTTCTCTGCCACGACTC
CCAAAAGGTTGGCCTTTGCGCCTCCTGACTGGATGGTATTGGATCTATTGCATACTTCTG
GTGGTTGCCTATCGGGCTTCATTAACCGCAATTTTGGCCAATCCTGTGGCTAGGGTTACC
ATCGACAAGCTGAAAGATCTCGCCGATAGCCCCATCCGCTGTGGAGCTTGGGGAGAACAG
AACAAATTATTCTTTCAAAGTGCTTCGGATCAAGTGTCCATGCAGATAGGGCAGAAGCTC
GAGCATACCCCCAAAGCGGAAGCCGCGGTCGAACGAGTCGTTGAAGGACATTTTGCGTAT
TATGACAACGTGTACATGTTGAAACATCTTCGGGCAACGCGAAAGTCTGCTAAAGCTCGC
GAAACGTTACACATCATGGAAGAATGTGCCGTCCACATGCCGATATCGATTGGACTGGAG
AAAAACTCACCGCTGAAACCAAAGGTGGACAAGTATGTTCGGGCCCTGGTGGAAGCTGGG
CTCACGAAGAAATGGCTTGCCGACGCCATTGAAGAGTTCCAGTCCAACGTCGAAATTCCC
CCACAAGAAGCTACGATGGACTTGCAAAAGCTAACGGCTGCCTTCATAGCACTGGCCATT
GGTTACGGCGTTTCACTGCTAGCCTTCAGCGCAGAGAAGCTGTACTGGCGGTTTGTGGTT
GAGAAGCATCCTGCCTATGATAAGTATATCGTTGGATCGTACCGCGGAAAGGTTGTGAGA
TTTTGA
4. Peptide sequence le
>AAEL005039:AAEL005039-RB peptide: AAEL005039-PB pep:protein_coding
MHTSTRGWMVRFFLISSFLSTLVESKPATNRSNFDDDNDLADFGHLLIDLVAKMKAGHCY
AFITDNIYQVVLTGRLFREIETFPRYVVQIPDYEDTLDPGTRVRCMLEEVRKIGCGAYVI
LLTNGIQMERLLRFGDRTRIINTRAKFIILHDYRLFVPDLHFLWKRIVNVVFVRQLVIDK
AKKRHESHHYELSTVPFPFPLRGVFISRRLDFWFNGKFRKGRKLFSDKTTNLDGQSMKVV
VLEHTPAIFRTTHNETDEHLKYYGLEVELLKAISEAMKFEMDFYESDDAAVAMWGTVTDG
ENATGLLGEMNEGHADFALADLHHTQYHLEIMDLSIPYNTECLTFLTPEALTDNSWTTLI
LPFTGGMWAGVLASLFSIGTVFYALSRLLMFVRHEQSYRSDLDMIAKRSRKRNRKVHFKI
VCGMVNSYKRALGNRKWLKFKKKSTDTSNMNKLKMLKMIPFKRQALPWKDPLPPRDIFDT
FSGCIIYTYSMLLLVSLPRLPKGWPLRLLTGWYWIYCILLVVAYRASLTAILANPVARVT
IDKLKDLADSPIRCGAWGEQNKLFFQSASDQVSMQIGQKLEHTPKAEAAVERVVEGHFAY
YDNVYMLKHLRATRKSAKARETLHIMEECAVHMPISIGLEKNSPLKPKVDKYVRALVEAG
LTKKWLADAIEEFQSNVEIPPQEATMDLQKLTAAFIALAIGYGVSLLAFSAEKLYWRFVV
EKHPAYDKYIVGSYRGKVVRF
5. Exon sequence le
>AAEL005039:AAEL005039-RB AAEL005039-RB-E1 exon:protein_coding
ATGCACACATCTACCAGAGGATGGATGGTCAGGTTTTTCCTGATAAGCAGTTTCTTGTCA
ACGCTGGTTGAATCCAAACCAGCGACTAATCGTTCCAATTTTGACGACGATAATGACCTC
GCCGATTTCGGGCACCTTTTGATCGATCTGGTTGCCAAGATGAAGGCTGGCCATTGCTAT
GCCTTCATCACGGATAATATCTACCAGGTTGTGTTAACGGGTCGTCTCTTCCGCGAGATT
GAAACCTTTCCACGTTACGTAGTTCAAATTCCGGACTATGAAGACACGCTAGATCCCGGA
ACACGTGTCCGGTGCATGCTCGAGGAAGTTCGGAAAATCGGTTGCGGGGCCTATGTTATA
TTGCTGACCAATGGGATCCAGATGGAGCGGTTGTTACGCTTTGGGGATAG
6. Screenshot from Blastn ortholog
7. Screenshot from tBlastn drosophila homolog
8. Screenshot of PFAM results
1
# Name
# SequenceGACAAGGATAATCAATACTCGCGCAAAGTTCATCATTCTCCACGACTATCGCCTGTTCGTGCCTGACCTGCATTTT
# Genome GCF_002204515.2
# PAM
NGG
# Position NC_035108.1:444454730-444455250:# Version CRISPOR 4.98, 2021-02-25T22:00:27CET
# Results http://crispor.org/crispor.py?batchId=OplalEznaiq3amLqAEBh
#guideId
276rev
346rev
470forw
40rev
182rev
471forw
154rev
289forw
315forw
390forw
465rev
52rev
62rev
80forw
227forw
285forw
326rev
345rev
379forw
472forw
189rev
196forw
487forw
176rev
86forw
342rev
117forw
188rev
380forw
384forw
477rev
151rev
303forw
502forw
187rev
253rev
357forw
235forw
459rev
67rev
215forw
140forw
132rev
267forw
511forw
171rev
250forw
411rev
432forw
327forw
targetSeq
CGATTGTCCGTCCAAGTTAGTGG
AATGCTCGTCCGTCTCATTGTGG
ATGATGCAGCCGTTGCCATGTGG
GCACGAACAGGCGATAGTCGTGG
AACACTCCTCTCAAGGGGAACGG
TGATGCAGCCGTTGCCATGTGGG
GAACGGTGCTTAGTTCGTAGTGG
GATAAGACCACTAACTTGGACGG
ATCGATGAAGGTCGTAGTGCTGG
GAAGTACTACGGGTTGGAAGTGG
GTCTGTTACGGTACCCCACATGG
AATGCAGGTCAGGCACGAACAGG
TTCCACAGAAAATGCAGGTCAGG
TGCCTGACCTGCATTTTCTGTGG
TTTCGCGAAGGCTCGATTTTTGG
CTCGGATAAGACCACTAACTTGG
TGGGTGGTTCTGAATATCGCCGG
ATGCTCGTCCGTCTCATTGTGGG
GACGAGCATTTGAAGTACTACGG
GATGCAGCCGTTGCCATGTGGGG
CGAAATAAACACTCCTCTCAAGG
CCGTTTCCGTTCCCCTTGAGAGG
ATGTGGGGTACCGTAACAGACGG
CCTCTCAAGGGGAACGGAAACGG
ACCTGCATTTTCTGTGGAAACGG
CTCGTCCGTCTCATTGTGGGTGG
TGTTGTTTTCGTCAGACAGCTGG
GAAATAAACACTCCTCTCAAGGG
ACGAGCATTTGAAGTACTACGGG
GCATTTGAAGTACTACGGGTTGG
AGCATTTTCGCCGTCTGTTACGG
CGGTGCTTAGTTCGTAGTGGTGG
CTTGGACGGACAATCGATGAAGG
ACAGACGGCGAAAATGCTACAGG
AAATAAACACTCCTCTCAAGGGG
TCTTATCCGAGAACAGTTTTCGG
CAGAACCACCCACAATGAGACGG
AGGCTCGATTTTTGGTTCAACGG
TACGGTACCCCACATGGCAACGG
TCCGTTTCCACAGAAAATGCAGG
GAGGAGTGTTTATTTCGCGAAGG
TGATTGACAAAGCCAAGAAAAGG
GTGGCTTTCATGCCTTTTCTTGG
AAAAGGCCGAAAACTGTTCTCGG
GAAAATGCTACAGGATTACTCGG
CAAGGGGAACGGAAACGGAACGG
TTCAACGGAAAATTTCGAAAAGG
CATTTCAAATTTCATTGCTTCGG
CGAAGCAATGAAATTTGAAATGG
CGTAGTGCTGGAGCATACACCGG
mitSpecScorecfdSpecScore
offtargetCounttargetGenomeGeneLocus
Doench ’16-Score
99
99
6 exon:XM_021839690.1 47
99
99
9 exon:XM_021839690.1 56
99
99
8 exon:XM_021839690.1 64
98
99
14 exon:XM_021839690.1 63
98
98
19 exon:XM_021839690.1 60
98
99
13 exon:XM_021839690.1 54
97
98
19 exon:XM_021839690.1 70
97
99
15 exon:XM_021839690.1 64
97
98
20 exon:XM_021839690.1 45
97
97
20 exon:XM_021839690.1 55
97
99
12 exon:XM_021839690.1 61
96
97
22 exon:XM_021839690.1 46
96
98
40 exon:XM_021839690.1 49
96
99
8 exon:XM_021839690.1 55
96
98
32 exon:XM_021839690.1 29
96
98
14 exon:XM_021839690.1 41
96
96
12 exon:XM_021839690.1 64
96
99
8 exon:XM_021839690.1 49
96
98
21 exon:XM_021839690.1 43
96
99
17 exon:XM_021839690.1 68
95
98
20 exon:XM_021839690.1 47
95
98
19 exon:XM_021839690.1 55
95
96
36 exon:XM_021839690.1 69
94
97
20 exon:XM_021839690.1 55
93
95
34 exon:XM_021839690.1 47
93
98
17 exon:XM_021839690.1 59
92
95
70 exon:XM_021839690.1 52
92
95
37 exon:XM_021839690.1 56
92
97
52 exon:XM_021839690.1 58
92
98
23 exon:XM_021839690.1 56
92
96
29 exon:XM_021839690.1 35
91
93
53 exon:XM_021839690.1 56
91
96
43 exon:XM_021839690.1 64
91
96
29 exon:XM_021839690.1 62
90
95
43 exon:XM_021839690.1 61
90
95
49 exon:XM_021839690.1 35
90
97
28 exon:XM_021839690.1 65
89
93
53 exon:XM_021839690.1 48
89
95
31 exon:XM_021839690.1 60
88
96
39 exon:XM_021839690.1 60
87
94
43 exon:XM_021839690.1 58
83
88
120 exon:XM_021839690.1 47
82
93
72 exon:XM_021839690.1 26
76
95
58 exon:XM_021839690.1 47
71
83
195 exon:XM_021839690.1 54
69
86
136 exon:XM_021839690.1 69
67
80
227 exon:XM_021839690.1 54
63
79
270 exon:XM_021839690.1 42
61
79
217 exon:XM_021839690.1 34
53
85
141 exon:XM_021839690.1 62
20210323030059exon2offtargetsites
2
TGTTCGTGCCTGACCTGCATTTTCTGTGGAAACGGATAGTAAATGTTGTTTTCGTCAGACAGCTGGTGATTGACAAAGCCAAGAAAAGGCATGAAAG
Moreno-Mateos-Score
Out-of-Frame-Score
Lindel-Score
40
63
89
36
67
81
55
49
85
30
57
74
44
66
66
40
43
79
34
68
83
30
66
81
24
52
65
42
53
71
53
55
78
49
54
72
54
49
71
26
78
76
34
63
74
42
64
89
56
71
83
57
69
77
33
52
74
76
43
87
10
65
84
63
69
84
74
44
75
49
64
72
46
67
70
50
67
86
7
57
80
34
62
80
49
54
62
35
67
82
43
47
73
60
53
70
42
55
74
32
62
67
37
64
72
27
76
82
30
58
68
60
72
84
59
44
77
22
60
78
42
70
86
44
77
79
18
71
81
36
76
83
30
58
72
67
85
74
33
66
68
22
54
80
17
55
71
65
69
83
GrafEtAlStatusgrafType
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
tt
GrafOK
GrafOK
tt
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
tt
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
GrafOK
tt
tt
GrafOK
GrafOK
GrafOK
tt
GrafOK
GrafOK
20210323030059exon2offtargetsites
3
CAAAGCCAAGAAAAGGCATGAAAGCCACCACTACGAACTAAGCACCGTTCCGTTTCCGTTCCCCTTGAGAGGAGTGTTTATTTCGCGAAGGCTCGA
20210323030059exon2offtargetsites
4
AGTGTTTATTTCGCGAAGGCTCGATTTTTGGTTCAACGGAAAATTTCGAAAAGGCCGAAAACTGTTCTCGGATAAGACCACTAACTTGGACGGACAAT
20210323030059exon2offtargetsites
5
AGACCACTAACTTGGACGGACAATCGATGAAGGTCGTAGTGCTGGAGCATACACCGGCGATATTCAGAACCACCCACAATGAGACGGACGAGCAT
20210323030059exon2offtargetsites
6
CCCACAATGAGACGGACGAGCATTTGAAGTACTACGGGTTGGAAGTGGAGCTGCTCAAAGCGATTTCCGAAGCAATGAAATTTGAAATGGATTTCT
20210323030059exon2offtargetsites
7
CAATGAAATTTGAAATGGATTTCTACGAAAGCGATGATGCAGCCGTTGCCATGTGGGGTACCGTAACAGACGGCGAAAATGCTACAGGATTACTCG
20210323030059exon2offtargetsites
8
TTGCCATGTGGGGTACCGTAACAGACGGCGAAAATGCTACAGGATTACTCGGAGAAATG
20210323030059exon2offtargetsites
>AAEL005039:AAEL005039-RB AAEL005039-RB-E1 exon:protein_coding
ATGCACACATCTACCAGAGGATGGATGGTCAGGTTTTTCCTGATAAGCAGTTTCTTGTCA
ACGCTGGTTGAATCCAAACCAGCGACTAATCGTTCCAATTTTGACGACGATAATGACCTC
GCCGATTTCGGGCACCTTTTGATCGATCTGGTTGCCAAGATGAAGGCTGGCCATTGCTAT
GCCTTCATCACGGATAATATCTACCAGGTTGTGTTAACGGGTCGTCTCTTCCGCGAGATT
GAAACCTTTCCACGTTACGTAGTTCAAATTCCGGACTATGAAGACACGCTAGATCCCGGA
ACACGTGTCCGGTGCATGCTCGAGGAAGTTCGGAAAATCGGTTGCGGGGCCTATGTTATA
TTGCTGACCAATGGGATCCAGATGGAGCGGTTGTTACGCTTTGGGGATAG
>AAEL005039:AAEL005039-RB AAEL005039-RB-E2 exon:protein_coding
GACAAGGATAATCAATACTCGCGCAAAGTTCATCATTCTCCACGACTATCGCCTGTTCGT
GCCTGACCTGCATTTTCTGTGGAAACGGATAGTAAATGTTGTTTTCGTCAGACAGCTGGT
GATTGACAAAGCCAAGAAAAGGCATGAAAGCCACCACTACGAACTAAGCACCGTTCCGTT
TCCGTTCCCCTTGAGAGGAGTGTTTATTTCGCGAAGGCTCGATTTTTGGTTCAACGGAAA
ATTTCGAAAAGGCCGAAAACTGTTCTCGGATAAGACCACTAACTTGGACGGACAATCGAT
GAAGGTCGTAGTGCTGGAGCATACACCGGCGATATTCAGAACCACCCACAATGAGACGGA
CGAGCATTTGAAGTACTACGGGTTGGAAGTGGAGCTGCTCAAAGCGATTTCCGAAGCAAT
GAAATTTGAAATGGATTTCTACGAAAGCGATGATGCAGCCGTTGCCATGTGGGGTACCGT
AACAGACGGCGAAAATGCTACAGGATTACTCGGAGAAATG
>AAEL005039:AAEL005039-RB AAEL005039-RB-E3 exon:protein_coding
AATGAAGGCCACGCTGACTTTGCGTTGGCTGATCTCCACCACACGCAATACCACTTGGAA
ATCATGGACCTGAGCATCCCGTACAACACCGAGTGTCTGACGTTCCTGACTCCGGAAGCG
CTGACCGACAATTCTTGGACGACGCTAATACTGCCTTTCACCGGAGGAATGTGGGCTGGC
GTCCTGGCATCGCTGTTTTCGATCGGAACCGTATTCTATGCCCTGTCGAGGCTTCTCATG
TTCGTACGCCACGAACAATCCTATCGCAGCGACTTGGATATGATTGCAAAAAGAAGTAGA
AAACGCAATCGGAAAGTTCATTTCAAGATAGTTTGTGGTATGGTGAATAGCTATAAGAGA
GCACTGGGTAACCGGAAATGGTTGAAGTTCAAGAAGAAATCGACGGATACCTCAAATATG
AACAAACTGAAAATGCTCAAAATGATTCCATTCAAAAGACAAGCTCTTCCTTGGAAAGAT
CCCCTGCCTCCCCGGGACATATTTGATACTTTCTCTGGATGTATCATCTATACGTATAGT
ATGCTTTTGCTAGTTTCTCTGCCACGACTCCCAAAAGGTTGGCCTTTGCGCCTCCTGACT
GGATGGTATTGGATCTATTGCATACTTCTGGTGGTTGCCTATCGGGCTTCATTAACCGCA
ATTTTGGCCAATCCTGTGGCTAGGGTTACCATCGACAAGCTGAAAGATCTCGCCGATAGC
CCCATCCGCTGTGGAGCTTGGGGAGAACAGAACAAATTATTCTTTCAAAGTGCTTCGGAT
CAAGTGTCCATGCAGATAGGGCAGAAGCTCGAGCATACCCCCAAAGCGGAAGCCGCGGTC
GAACGAGTCGTTGAAGGACATTTTGCGTATTATGACAACGTGTACATGTTGAAACATCTT
CGGGCAACGCGAAAGTCTGCTAAAGCTCGCGAAACGTTACACATCATGGAAGAATGTGCC
GTCCACATGCCGATATCGATTGGACTGGAGAAAAACTCACCGCTGAAACCAAAGGTGGAC
AAGTATGTTCGGGCCCTGGTGGAAGCTGGGCTCACGAAGAAATGGCTTGCCGACGCCATT
GAAGAGTTCCAGTCCAACGTCGAAATTCCCCCACAAGAAGCTACGATGGACTTGCAAAAG
CTAACGGCTGCCTTCATAGCACTGGCCATTGGTTACGGCGTTTCACTGCTAGCCTTCAGC
GCAGAGAAGCTGTACTGGCGGTTTGTGGTTGAGAAGCATCCTGCCTATGATAAGTATATC
GTTGGATCGTACCGCGGAAAGGTTGTGAGATTTTGAAGGAATGATCTTTAAGGAATAAAG
TA
>2 dna:chromosome chromosome:AaegL5:2:444452977:444455726:-1
ATGCACACATCTACCAGAGGATGGATGGTCAGGTTTTTCCTGATAAGCAGTTTCTTGTCA
ACGCTGGTTGAATCCAAACCAGCGACTAATCGTTCCAATTTTGACGACGATAATGACCTC
GCCGATTTCGGGCACCTTTTGATCGATCTGGTTGCCAAGATGAAGGCTGGCCATTGCTAT
GCCTTCATCACGGATAATATCTACCAGGTTGTGTTAACGGGTCGTCTCTTCCGCGAGATT
GAAACCTTTCCACGTTACGTAGTTCAAATTCCGGACTATGAAGACACGCTAGATCCCGGA
ACACGTGTCCGGTGCATGCTCGAGGAAGTTCGGAAAATCGGTTGCGGGGCCTATGTTATA
TTGCTGACCAATGGGATCCAGATGGAGCGGTTGTTACGCTTTGGGGATAGGTGAGAGTAA
ATAGGTTCAAAAATTTCTTGAACTAGGTCCTATTTATTCCTTCTCGTTGAATTTAGGACA
AGGATAATCAATACTCGCGCAAAGTTCATCATTCTCCACGACTATCGCCTGTTCGTGCCT
GACCTGCATTTTCTGTGGAAACGGATAGTAAATGTTGTTTTCGTCAGACAGCTGGTGATT
GACAAAGCCAAGAAAAGGCATGAAAGCCACCACTACGAACTAAGCACCGTTCCGTTTCCG
TTCCCCTTGAGAGGAGTGTTTATTTCGCGAAGGCTCGATTTTTGGTTCAACGGAAAATTT
CGAAAAGGCCGAAAACTGTTCTCGGATAAGACCACTAACTTGGACGGACAATCGATGAAG
GTCGTAGTGCTGGAGCATACACCGGCGATATTCAGAACCACCCACAATGAGACGGACGAG
CATTTGAAGTACTACGGGTTGGAAGTGGAGCTGCTCAAAGCGATTTCCGAAGCAATGAAA
TTTGAAATGGATTTCTACGAAAGCGATGATGCAGCCGTTGCCATGTGGGGTACCGTAACA
GACGGCGAAAATGCTACAGGATTACTCGGAGAAATGGTATGGATTGAATTGAATCACTAC
CATTGAAATGAGTAGAATCTACCGAATCATAACTTTTCAAAGCTTATGGGCAGAAAATTG
CATTTTAGAATATTTACTTATATAATTAACATCTAAACAGATAACACTGAATTTCGCGCC
ACAAAGCTCGATTTGTGGCCATATTTATTTATTTAGTTCGTAAATCAAAAGTAGACTACA
TTTTAACACTTGAATATATATACTACTATATCTCACAGAGTTAAAGTATAATCTTAAGTT
ACTACGCGTCATTTTTCATATTCATAACATAACTCGTTTGAATTGCATACTGATTGTCAA
TTACAACTTGTGCTGTATAAAAATCCATTCTGCATCTTGTCGCGCGAAACAATTTTTTAT
ACAAAGATGAGCCACAAATTTCTTCATTGTTTTAACTATGTTTTCCAGAATGAAGGCCAC
GCTGACTTTGCGTTGGCTGATCTCCACCACACGCAATACCACTTGGAAATCATGGACCTG
AGCATCCCGTACAACACCGAGTGTCTGACGTTCCTGACTCCGGAAGCGCTGACCGACAAT
TCTTGGACGACGCTAATACTGCCTTTCACCGGAGGAATGTGGGCTGGCGTCCTGGCATCG
CTGTTTTCGATCGGAACCGTATTCTATGCCCTGTCGAGGCTTCTCATGTTCGTACGCCAC
GAACAATCCTATCGCAGCGACTTGGATATGATTGCAAAAAGAAGTAGAAAACGCAATCGG
AAAGTTCATTTCAAGATAGTTTGTGGTATGGTGAATAGCTATAAGAGAGCACTGGGTAAC
CGGAAATGGTTGAAGTTCAAGAAGAAATCGACGGATACCTCAAATATGAACAAACTGAAA
ATGCTCAAAATGATTCCATTCAAAAGACAAGCTCTTCCTTGGAAAGATCCCCTGCCTCCC
CGGGACATATTTGATACTTTCTCTGGATGTATCATCTATACGTATAGTATGCTTTTGCTA
GTTTCTCTGCCACGACTCCCAAAAGGTTGGCCTTTGCGCCTCCTGACTGGATGGTATTGG
ATCTATTGCATACTTCTGGTGGTTGCCTATCGGGCTTCATTAACCGCAATTTTGGCCAAT
CCTGTGGCTAGGGTTACCATCGACAAGCTGAAAGATCTCGCCGATAGCCCCATCCGCTGT
GGAGCTTGGGGAGAACAGAACAAATTATTCTTTCAAAGTGCTTCGGATCAAGTGTCCATG
CAGATAGGGCAGAAGCTCGAGCATACCCCCAAAGCGGAAGCCGCGGTCGAACGAGTCGTT
GAAGGACATTTTGCGTATTATGACAACGTGTACATGTTGAAACATCTTCGGGCAACGCGA
AAGTCTGCTAAAGCTCGCGAAACGTTACACATCATGGAAGAATGTGCCGTCCACATGCCG
ATATCGATTGGACTGGAGAAAAACTCACCGCTGAAACCAAAGGTGGACAAGTATGTTCGG
GCCCTGGTGGAAGCTGGGCTCACGAAGAAATGGCTTGCCGACGCCATTGAAGAGTTCCAG
TCCAACGTCGAAATTCCCCCACAAGAAGCTACGATGGACTTGCAAAAGCTAACGGCTGCC
TTCATAGCACTGGCCATTGGTTACGGCGTTTCACTGCTAGCCTTCAGCGCAGAGAAGCTG
TACTGGCGGTTTGTGGTTGAGAAGCATCCTGCCTATGATAAGTATATCGTTGGATCGTAC
CGCGGAAAGGTTGTGAGATTTTGAAGGAATGATCTTTAAGGAATAAAGTA
>AAEL005039:AAEL005039-RB AAEL005039-RB-E1 exon:protein_coding
ATGCACACATCTACCAGAGGATGGATGGTCAGGTTTTTCCTGATAAGCAGTTTCTTGTCA
ACGCTGGTTGAATCCAAACCAGCGACTAATCGTTCCAATTTTGACGACGATAATGACCTC
GCCGATTTCGGGCACCTTTTGATCGATCTGGTTGCCAAGATGAAGGCTGGCCATTGCTAT
GCCTTCATCACGGATAATATCTACCAGGTTGTGTTAACGGGTCGTCTCTTCCGCGAGATT
GAAACCTTTCCACGTTACGTAGTTCAAATTCCGGACTATGAAGACACGCTAGATCCCGGA
ACACGTGTCCGGTGCATGCTCGAGGAAGTTCGGAAAATCGGTTGCGGGGCCTATGTTATA
TTGCTGACCAATGGGATCCAGATGGAGCGGTTGTTACGCTTTGGGGATAG
>AAEL005039:AAEL005039-RB AAEL005039-RB-E2 exon:protein_coding
GACAAGGATAATCAATACTCGCGCAAAGTTCATCATTCTCCACGACTATCGCCTGTTCGT
GCCTGACCTGCATTTTCTGTGGAAACGGATAGTAAATGTTGTTTTCGTCAGACAGCTGGT
GATTGACAAAGCCAAGAAAAGGCATGAAAGCCACCACTACGAACTAAGCACCGTTCCGTT
TCCGTTCCCCTTGAGAGGAGTGTTTATTTCGCGAAGGCTCGATTTTTGGTTCAACGGAAA
ATTTCGAAAAGGCCGAAAACTGTTCTCGGATAAGACCACTAACTTGGACGGACAATCGAT
GAAGGTCGTAGTGCTGGAGCATACACCGGCGATATTCAGAACCACCCACAATGAGACGGA
CGAGCATTTGAAGTACTACGGGTTGGAAGTGGAGCTGCTCAAAGCGATTTCCGAAGCAAT
GAAATTTGAAATGGATTTCTACGAAAGCGATGATGCAGCCGTTGCCATGTGGGGTACCGT
AACAGACGGCGAAAATGCTACAGGATTACTCGGAGAAATG
>AAEL005039:AAEL005039-RB AAEL005039-RB-E3 exon:protein_coding
AATGAAGGCCACGCTGACTTTGCGTTGGCTGATCTCCACCACACGCAATACCACTTGGAA
ATCATGGACCTGAGCATCCCGTACAACACCGAGTGTCTGACGTTCCTGACTCCGGAAGCG
CTGACCGACAATTCTTGGACGACGCTAATACTGCCTTTCACCGGAGGAATGTGGGCTGGC
GTCCTGGCATCGCTGTTTTCGATCGGAACCGTATTCTATGCCCTGTCGAGGCTTCTCATG
TTCGTACGCCACGAACAATCCTATCGCAGCGACTTGGATATGATTGCAAAAAGAAGTAGA
AAACGCAATCGGAAAGTTCATTTCAAGATAGTTTGTGGTATGGTGAATAGCTATAAGAGA
GCACTGGGTAACCGGAAATGGTTGAAGTTCAAGAAGAAATCGACGGATACCTCAAATATG
AACAAACTGAAAATGCTCAAAATGATTCCATTCAAAAGACAAGCTCTTCCTTGGAAAGAT
CCCCTGCCTCCCCGGGACATATTTGATACTTTCTCTGGATGTATCATCTATACGTATAGT
ATGCTTTTGCTAGTTTCTCTGCCACGACTCCCAAAAGGTTGGCCTTTGCGCCTCCTGACT
GGATGGTATTGGATCTATTGCATACTTCTGGTGGTTGCCTATCGGGCTTCATTAACCGCA
ATTTTGGCCAATCCTGTGGCTAGGGTTACCATCGACAAGCTGAAAGATCTCGCCGATAGC
CCCATCCGCTGTGGAGCTTGGGGAGAACAGAACAAATTATTCTTTCAAAGTGCTTCGGAT
CAAGTGTCCATGCAGATAGGGCAGAAGCTCGAGCATACCCCCAAAGCGGAAGCCGCGGTC
GAACGAGTCGTTGAAGGACATTTTGCGTATTATGACAACGTGTACATGTTGAAACATCTT
CGGGCAACGCGAAAGTCTGCTAAAGCTCGCGAAACGTTACACATCATGGAAGAATGTGCC
GTCCACATGCCGATATCGATTGGACTGGAGAAAAACTCACCGCTGAAACCAAAGGTGGAC
AAGTATGTTCGGGCCCTGGTGGAAGCTGGGCTCACGAAGAAATGGCTTGCCGACGCCATT
GAAGAGTTCCAGTCCAACGTCGAAATTCCCCCACAAGAAGCTACGATGGACTTGCAAAAG
CTAACGGCTGCCTTCATAGCACTGGCCATTGGTTACGGCGTTTCACTGCTAGCC…
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