ACID FAST?
YES
NO
(3pts)
IDENTIFICATION
Genus
Specie (Extra Points)
.
WRITE A PARAGRAPH (10 – 20 informative sentences,
type written) about the significance of the bacterium
you identified, as it relates to humans in healthy or
diseased state (e.g. medical importance, use in food
production or biotechnology (23pts)
There are several books in the library that can help you
with this portion of the report.
Rubric for grading the write-up:
Scientific spelling/Scientific nomenclature (5
points)
Content: (10 points)
• Citation of reference, both in text and listed
at the end of write-up (8 points)
WEEBSITES THAT YOU CAN TRUST: Please do not
copy, but state in your own words (No Plagiarism).
• http//www.cdc.gov/az/
http://www.fda.gov/Sitelndex/default.htm
• http://textbookofbacteriology.net/index.html
dering
NB: Please use the Writing Center (Resource Center-RC
105). They can help you organize information and
improve your writing skills.
GRADING GRAM STAIN (8 points total)
Rubric for grading Gram stain:
• Looking for healthy cells (2pts), right color (3pts) &
ability to focus (3pts)
GRAM REACTION (POSITIVE OR NEGATIVE): (1 pt)
20
SHAPE: REFER TO CHAPTER 4 LECTURE NOTES: (3pts)
ortar
ARRANGEMENT: (REFER TO CHAPTER 4 LECTURE
NOTES): (3pts)
por
APPEARANCE OF ORGANISMS ON SLIDE: YOUR
DESCRIPTION SHOULD BE BASED ON YOUR
OBSERVATION: (1 pt)
boed
IS YOUR ORGANISM:
NO
Motile?
YES
CIRCLE ONE (1 pt)
DOES YOUR ORGANISM MAKE (CIRCLE ONE)
ENDOSPORES?
YES
NO
(3pts)
CAPSULES?
YES
NO
(3pts)
Materials Neededoint)
1. Slides
2. Staining Trays and Reagents
3. Forceps
4. Clothespins
5. Stains
Cultures of: One of these organisms below is yours
1. Staphylococcus epidermidis
2. Staphylococcus aureus
3. Enterococcus fecalis
4. Micrococcus luteus
5. Bacillus subtilis FR TO CHAPTER LECTOR
6. Eschericia coli
7. Klebsiella pnuemoniae
8. Enterobacter aerogenes
UNKNOWN WORKSHEET TO BE GRADED. PLEASE DO
NOT RETYPE. YOU WILL LOOSE 5PTS, IF YOU DO!!!!
STUDENT NAME:
UNKNOWN #
CLASS LABORATORY SECTION
DESCRIPTION OF BROTH CHARACTERISTICS: (1 pt)
BLIND CHASE
THE MORPHOLOGIC UNKNOWN
SPRING 2022
O
Purpose of Activity: To introduce Staining as a standard
practice for bacterial identifications
Type of Activity: Hands-on
Review/Reinforcement
Types of Skills Emphasized:
• Good Observation
Analytical/Reasoning
• Basic Research Techniques
Aseptic Techniques
Staining Techniques
• Identification of Gram Reaction, Shapes and
Arrangements
• Identification of special structures using special stains
(Spores & Capsules)
• Description of Broth, Characteristics)
• Identification of Unknown (Genus & Specie not necessary
at this point and will be extra credit, if correct)
• Basic Research Techniques
This exercise requires you to show that you have mastered
the methods above that were taught during the earlier
laboratory activities.
For the tips and hints that will enhance your successful
completion of this module, please go to page 23 of the
General Microbiology: A laboratory Study Guide by Dr.
Ijeoma Onwenu-Otigbuo. This study Guide accompanies the
Laboratory book and is optional
Course: Hou…
6 COVID-19 Visualizer
eg Used Land Rover R… America
e-coli in nutrient broth
E. coli
seudomonas aeruginosa growth on Thioglycollate hroth
Lab 4 Specimen
Corynebacterium
merosis: Gram-
postitive bacilli
commonly found on
skin, rarely causes
Infection, can cause
endocarditis, skin
infections, other
illnesses.
Escherichia
Gram-nega
facultative
anaerobic,
shaped bac
commonly
the lower i
warm-bloo
organisms.
Staphylococcus
epidermidis: Gram-
positive staphylo-
cocci in skin,
mucosal flora and is
harmful to immuno-
compromised
patients
Mycobacter
smegmatis
bacilli found
syphilitic an
genital secr
is generally
pathogenic,
Very rare ca
>I
25:28 / 28:07 – Lab 4 Specimen >
4: Bacterial Structure, Simple Stains, Negative Stains, Gram & Acid-Fast Stains
S. Jun 8, 2019
$ 219
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