RUBRIC
HPLC-IC Lab
Section
Title (1 pt)
Abstract (8 pts)
Introduction(26 pts)
Methods (2 pt)
Results & Discussion (31 pts)
Formatting (7 pts)
Conclusion (6 pts)
Excel (15 pts)
References (4 pts)
TOTAL POINTS
Points Awarded For
Descriptive but concise title
Purpose statement
Methods statement
All final results
Short, concise, but complete summary
Purpose statement
Theory of HPLC and IC
Real-world use/application of HPLC and IC
Importance of Linalool and Lavendar Oil
Importance of cations (Na+, K+, Mg2+, Ca2+)
Does not include methodology, only theory
Multiple in-text citations, correctly formatted
Reference Canvas Modules
Properly Labelled chromatograms of Lavendar and Linalool for each method
Rationale for method selection
Correct Calibration curve of Linalool in Lavendar Oil sample
Calculation of Linalool concentration in Lavendar Oil
Rationale for choosing the standards concentration in IC
Calibration curves for the 4 cations standards in IC
Value of unknown and calculations in IC
Importance/Implication of results
All figures/tables correctly formatted
Third person, passive voice, past tense, single spaced, 12 pt, justified
Does not just data dump
Implications for scientific community
Repeat the main take-aways (the purposes and the results)
Clearly labelled, coded, matches report
References section, ACS style
Points Possible
1
2
2
2
2
2
6
4
4
4
2
4
2
6
2
4
4
2
6
4
3
3
3
1
3
3
15
4
100
Points Awarded
Page 1-2
3/5/2022 9:46 PM
Operator:mtessman Timebase:IC1000 Sequence:IC Cation Runs
7
Blank(MilliQ2 Run2)
Sample Name:
Vial Number:
Sample Type:
Control Program:
Quantif. Method:
Recording Time:
Run Time (min):
0.300
Blank(MilliQ2 Run2)
8
standard
Cations
Scott
10/12/2016 15:36
12.74
Injection Volume:
Channel:
Wavelength:
Bandwidth:
Dilution Factor:
Sample Weight:
Sample Amount:
IC Cation Runs #7 [modif ied by acs-chem-adm]
µS
20.0
ECD_1
n.a.
n.a.
1.0000
1.0000
1.0000
ECD_1
5 – Sodium – 3.337
0.200
0.100
8 – Calcium – 9.883
6 – Potassium – 5.003
7 – Magnesium – 7.717
-0.000
12-3-2.567
4-2.700
-2.853
2.983
-0.100
0.0
No.
1
2
3
4
5
6
7
8
Total:
Ret.Time
min
2.57
2.70
2.85
2.98
3.34
5.00
7.72
9.88
min
2.0
4.0
Peak Name
n.a.
n.a.
n.a.
n.a.
Sodium
Potassium
Magnesium
Calcium
20220305202435ic_data_blank_milliq/Integration
6.0
Height
µS
0.000
0.007
0.004
0.004
0.277
0.089
0.027
0.101
0.510
8.0
10.0
Area
Rel.Area
µS*min
%
0.000
0.01
0.002
1.00
0.000
0.28
0.001
0.45
0.061
39.55
0.025
16.41
0.010
6.70
0.055
35.60
0.154 100.00
12.7
Amount
n.a.
n.a.
n.a.
n.a.
0.058
0.041
0.009
0.044
0.153
Type
RuAv*
MAv*
MAv*
MAv*
MAv*
MAv*
MAv*
MAv*
Chromeleon (c) Dionex 1996-2006
Version 6.80 SR15 Build 4656 (243203)
Operator:mtessman Timebase:IC1000 Sequence:IC Cation Runs
Page 2-2
3/5/2022 9:46 PM
ECD_1
8 – Calcium – 9.883
7.717
10.0
min
12.7
20220305202435ic_data_blank_milliq/Integration
Chromeleon (c) Dionex 1996-2006
Version 6.80 SR15 Build 4656 (243203)
Page 1-2
3/5/2022 9:42 PM
Operator:mtessman Timebase:IC1000 Sequence:IC Cation Runs
5
Std5(1:5)
Sample Name:
Vial Number:
Sample Type:
Control Program:
Quantif. Method:
Recording Time:
Run Time (min):
Std5(1:5)
6
standard
Cations
Scott
10/12/2016 14:56
12.11
Injection Volume:
Channel:
Wavelength:
Bandwidth:
Dilution Factor:
Sample Weight:
Sample Amount:
8.00 IC Cation Runs #5
µS
Std5(1:5)
20.0
ECD_1
n.a.
n.a.
1.0000
1.0000
1.0000
ECD_1
5 – Sodium – 3.330
6.25
5.00
8 – Magnesium – 7.650
3.75
6 – Potassium – 4.970
10 – Calcium – 9.770
2.50
1.25
7 – 6.710
12-3-2.620
4-2.727
-2.843
2.983
-1.00
0.0
No.
1
2
3
4
5
6
7
8
9
10
Total:
9 – 8.847
min
1.3
Ret.Time
min
2.62
2.73
2.84
2.98
3.33
4.97
6.71
7.65
8.85
9.77
2.5
3.8
Peak Name
n.a.
n.a.
n.a.
n.a.
Sodium
Potassium
n.a.
Magnesium
n.a.
Calcium
20220305202435ic_data_standard_2_ppm/Integration
5.0
6.3
Height
µS
0.001
0.001
0.001
0.002
6.735
3.188
0.013
3.673
0.015
2.814
16.442
7.5
8.8
Area
Rel.Area
µS*min
%
0.000
0.00
0.000
0.00
0.000
0.00
0.000
0.00
1.216
26.73
0.740
16.27
0.007
0.16
1.257
27.64
0.005
0.11
1.322
29.07
4.548 100.00
10.0
Amount
n.a.
n.a.
n.a.
n.a.
1.165
1.189
n.a.
1.127
n.a.
1.072
4.554
12.1
Type
BM
M
M
Mb
bMB
BM
BMb
bM
Mb
bMB
Chromeleon (c) Dionex 1996-2006
Version 6.80 SR15 Build 4656 (243203)
Operator:mtessman Timebase:IC1000 Sequence:IC Cation Runs
Page 2-2
3/5/2022 9:42 PM
ECD_1
– 7.650
10 – Calcium – 9.770
.847
min
10.0
12.1
20220305202435ic_data_standard_2_ppm/Integration
Chromeleon (c) Dionex 1996-2006
Version 6.80 SR15 Build 4656 (243203)
Page 1-2
3/5/2022 9:42 PM
Operator:mtessman Timebase:IC1000 Sequence:IC Cation Runs
6
Std6(1:1)
Sample Name:
Vial Number:
Sample Type:
Control Program:
Quantif. Method:
Recording Time:
Run Time (min):
Std6(1:1)
7
standard
Cations
Scott
10/12/2016 15:20
11.96
Injection Volume:
Channel:
Wavelength:
Bandwidth:
Dilution Factor:
Sample Weight:
Sample Amount:
40.0 IC Cation Runs #6
µS
Std6(1:1)
20.0
ECD_1
n.a.
n.a.
1.0000
1.0000
1.0000
ECD_1
5 – Sodium – 3.333
30.0
8 – Magnesium – 7.553
20.0
6 – Potassium – 4.943
10 – Calcium – 9.627
10.0
7 – 6.703
12-3-42.627
-2.737
-2.840
2.907
-5.0
0.0
No.
1
2
3
4
5
6
7
8
9
10
Total:
9 – 8.753
min
1.3
Ret.Time
min
2.63
2.74
2.84
2.91
3.33
4.94
6.70
7.55
8.75
9.63
2.5
3.8
5.0
Peak Name
n.a.
n.a.
n.a.
n.a.
Sodium
Potassium
n.a.
Magnesium
n.a.
Calcium
20220305202436ic_data_standard_10_ppm/Integration
6.3
Height
µS
0.001
0.001
0.001
0.001
35.022
16.879
0.050
21.191
0.020
14.706
87.872
7.5
8.8
Area
Rel.Area
µS*min
%
0.000
0.00
0.000
0.00
0.000
0.00
0.000
0.00
6.027
25.31
3.694
15.51
0.031
0.13
7.301
30.66
0.005
0.02
6.756
28.37
23.814 100.00
10.0
Amount
n.a.
n.a.
n.a.
n.a.
5.776
5.935
n.a.
6.548
n.a.
5.477
23.735
12.0
Type
BM
M
Mb
bMB
bMB
BM
BMB
bMb
bMb
bMB
Chromeleon (c) Dionex 1996-2006
Version 6.80 SR15 Build 4656 (243203)
Operator:mtessman Timebase:IC1000 Sequence:IC Cation Runs
Page 2-2
3/5/2022 9:42 PM
ECD_1
7.553
10 – Calcium – 9.627
.753
min
10.0
12.0
20220305202436ic_data_standard_10_ppm/Integration
Chromeleon (c) Dionex 1996-2006
Version 6.80 SR15 Build 4656 (243203)
Page 1-2
3/5/2022 9:25 PM
Operator:mtessman Timebase:IC1000 Sequence:IC Cation Runs
2
Std2(1:625)
Sample Name:
Vial Number:
Sample Type:
Control Program:
Quantif. Method:
Recording Time:
Run Time (min):
Std2(1:625)
3
standard
Cations
Scott
10/12/2016 14:04
15.00
Injection Volume:
Channel:
Wavelength:
Bandwidth:
Dilution Factor:
Sample Weight:
Sample Amount:
0.500 IC Cation Runs #2
µS
Std2(1:625)
20.0
ECD_1
n.a.
n.a.
1.0000
1.0000
1.0000
ECD_1
8 – Sodium – 3.330
0.400
0.300
0.200
10 – Potassium – 4.993
12 – Calcium – 9.897
0.100
11 – Magnesium – 7.710
9 – 3.833
1234-5
7
-2.927
3.043
–6-2.570
2.653
2.747
– 2.857
-2.703
-0.000
-0.100
0.0
No.
1
2
3
4
5
6
7
8
9
10
11
12
Total:
Ret.Time
min
2.57
2.65
2.70
2.75
2.86
2.93
3.04
3.33
3.83
4.99
7.71
9.90
min
2.0
4.0
6.0
Peak Name
n.a.
n.a.
n.a.
n.a.
n.a.
n.a.
n.a.
Sodium
n.a.
Potassium
Magnesium
Calcium
20220305202436ic_data_standard_16_ppb/Integration
Height
µS
0.000
0.000
0.000
0.000
0.002
0.002
0.000
0.454
0.028
0.172
0.046
0.130
0.834
8.0
10.0
12.0
Area
Rel.Area
µS*min
%
0.000
0.01
0.000
0.00
0.000
0.00
0.000
0.00
0.000
0.08
0.000
0.06
0.000
0.01
0.080
36.85
0.006
2.88
0.043
19.95
0.018
8.43
0.069
31.73
0.217 100.00
Amount
n.a.
n.a.
n.a.
n.a.
n.a.
n.a.
n.a.
0.077
n.a.
0.070
0.016
0.056
0.219
15.0
Type
BMB
BMb
bMb
bMb
bM
M
MB
BM
MB
M
BMB
BMB
Chromeleon (c) Dionex 1996-2006
Version 6.80 SR15 Build 4656 (243203)
Operator:mtessman Timebase:IC1000 Sequence:IC Cation Runs
Page 2-2
3/5/2022 9:25 PM
ECD_1
m – 9.897
min
12.0
15.0
20220305202436ic_data_standard_16_ppb/Integration
Chromeleon (c) Dionex 1996-2006
Version 6.80 SR15 Build 4656 (243203)
Page 1-2
3/5/2022 9:25 PM
Operator:mtessman Timebase:IC1000 Sequence:IC Cation Runs
3
Std3(1:125)
Sample Name:
Vial Number:
Sample Type:
Control Program:
Quantif. Method:
Recording Time:
Run Time (min):
Std3(1:125)
4
standard
Cations
Scott
10/12/2016 14:21
15.00
Injection Volume:
Channel:
Wavelength:
Bandwidth:
Dilution Factor:
Sample Weight:
Sample Amount:
0.600 IC Cation Runs #3
µS
Std3(1:125)
20.0
ECD_1
n.a.
n.a.
1.0000
1.0000
1.0000
ECD_1
4 – Sodium – 3.330
0.500
0.400
0.300
0.200
8 – Calcium – 9.870
6 – Potassium – 4.987
7 – Magnesium – 7.710
0.100
5 – 3.823
– -2.843
2.953
1 -232.583
-0.000
-0.100
0.0
No.
1
2
3
4
5
6
7
8
Total:
Ret.Time
min
2.58
2.84
2.95
3.33
3.82
4.99
7.71
9.87
min
2.0
4.0
6.0
Peak Name
n.a.
n.a.
n.a.
Sodium
n.a.
Potassium
Magnesium
Calcium
20220305202506ic_data_standard_80_ppb/Integration
Height
µS
0.001
0.000
0.000
0.550
0.034
0.176
0.110
0.174
1.046
8.0
10.0
12.0
Area
Rel.Area
µS*min
%
0.000
0.02
0.000
0.01
0.000
0.00
0.099
35.16
0.007
2.62
0.045
16.11
0.040
14.35
0.089
31.72
0.282 100.00
Amount
n.a.
n.a.
n.a.
0.095
n.a.
0.073
0.036
0.072
0.276
15.0
Type
BMB
BMB
BMB
BM
MB
M
BMB
BMB
Chromeleon (c) Dionex 1996-2006
Version 6.80 SR15 Build 4656 (243203)
Operator:mtessman Timebase:IC1000 Sequence:IC Cation Runs
Page 2-2
3/5/2022 9:25 PM
ECD_1
– 9.870
min
12.0
15.0
20220305202506ic_data_standard_80_ppb/Integration
Chromeleon (c) Dionex 1996-2006
Version 6.80 SR15 Build 4656 (243203)
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