complete only the highlighted part of attachment below
one attachment is the actual post lab, the other is the graphs taken and used for calculation, simply do the highlighted part. Make sure where it says show handwritten work to do hand written work
Name:
Experiment 5 – Investigating Gas Chromatography with the SeaPORT Mini-GC
a) Introduction/purpose
b) Experimental
Table 1: Reagents
Compound
MM
(g/mol)
Formula
BP ( oC)
Density
(g/mL)
Health
Hazard
Amount
Structure
Instrument
GC model
Column type
Carrier gas
Detector type
Supporting software
c) Data and Results:
GC parameters tested:
Table 2 – GC Parameters
Starting Temp
(oC)
Hold Time
(min)
Ramp Rate
(oC/min)
Final Temp
(oC)
Hold Time
(min)
Total Run
Length (min)
Pressure
(kPa)
Run 1
Run 2
Run 3
Run 4
Run 5
Run 6
120
100
70
70
50
40
6
0
0
0
0
0
0
0
0
120
100
70
0
0
6
20
Run 7
Run 8
Run 9
40
40
40
40
0
1.0
1.0
1.0
1.0
0
0
10
10
10
10
70
59
40
120
120
120
0
0
0
0
0
0
0
0
5
10
10
10
10
10
10
10
10
15
10
8
8
8
10
15
18
20
120
Run 10
Note: Include chromatograms (Figures 1 – 10) with hand measured baseline widths (Wb) of the first two
peaks (use a ruler!). Write the run number and GC parameters directly on the chromatogram. Label each
chromatogram peak. Include all chromatograms with the write-up in consecutive order. (Note: you may
have less than 10 trials if you find the optimal parameters with fewer runs)
d) Discussion
Discuss an effective strategy for optimizing GC parameters for the selection of your best parameters.
1
Experiment 5
Name:
e) Question Responses:
Question #1: What is the resolution between the two closest peaks in each chromatogram? Show
your calculations on the actual chromatograms and a sample calculation for determining
resolution.
Table 3 – Effect of GC conditions on peak resolution
Run
tR (min) Peak 1
tR (min) Peak 2
Wb (min) Peak 1 Wb (min) Peak 2
Resolution
1
2
3
4
5
6
7
8
9
10
Note: You MUST show your work for at least one the resolution calculation for full credit.
Question #2: The initial run is isocratic, but an ideal run will actually be a gradient run. Why is
this needed to achieve ideal separation?
Question #3: What parameter had the greatest effect on the peak shape and separation? Explain
why you chose this parameter.
Question #4: In what order did the molecules elute from the column? Describe the stepwise
protocol you followed in this determination. (Include any chromatograms employed labeled with
temperature and pressure conditions)
Question #5: The mixture composition was in the ratio of 4:4:1 propyl acetate: ethyl acetate: ethyl
ethoxyacetate. Based on that ratio, compare the relative sensitivity of the detector to each
component?
Question #6: Based on your data collected, which parameters gave the best separation? Why do
you consider this the best?
2
Experiment 5
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